摘要
目的研究1999～2003年我院真菌性角膜炎的病原学分布。方法回顾性分析我院化脓性角膜炎真菌涂片标本922份、连续性真菌培养标本1814份,并与我院1975～1986、1989～1997年两个阶段比较。结果涂片标本阳性率为27%(306/922),与培养的阳性一致率为82%;真菌培养阳性率为25%(456/1814);上半年与下半年的比例为1:2.2;培养阳性的真菌菌株中,镰孢菌属占34%(155/456),曲霉菌属占26%(118/456);茄病镰孢菌和烟曲霉菌分别是最主要的致病菌株;与我院前两个阶段的资料比较曲霉菌的比例有明显下降而镰孢菌有明显上升的趋势;另外,456份标本中80份伴有细菌培养阳性。结论目前,镰孢菌属和曲霉菌属是广州地区真菌性角膜炎的主要致病真菌。
Objective Report the pathogenic features of fungal keratitis diagnosed in Zhongshan Ophthalmic Center in Guangzhou between 1999 and 2003.Methods We retrospectively reviewed 922 corneal smears and 1814 consecutive fungal culture results of purulent keratitis.Compare them with data of 1975-1986 and 1989-1997 of our hospital.Results 27%(306/922)of the corneal scraping smear examination for fungi is positive,and 82% of them were verified by later culture.Positive rate of fungal culture in our hospital was 25% during 1999-2003.More cases of fungal keratitis were found in the second half of year(from July to December)than the first half(from January to June),and the ratio of them was 1∶2.2.Fusarium species were most commonly isolated 34%(155/456),followed by Aspergillus species 26%(118/456)and Curvularia 14%(64/456).Fusarium solani was most frequently found in Fusarium species and Aspergillus fumigatus was most frequently found in Aspergillus species.The comparison of the structure of pathogenic fungi,we found an increasing trend of Fusarium species and a decreasing trend of Aspergillus species.Besides,80 specimens were cultured positive both bacteria and fungi,with Staphylococcus epidermidis 50%.Conclusions Fusarium species and Aspergillus species are the most frequent pathogenic fungi in the area of Guangzhou.
出处
《中国实用眼科杂志》
CSCD
北大核心
2005年第5期506-508,共3页
Chinese Journal of Practical Ophthalmology
