摘要
背景与目的:树突细胞(dendriticcells,DCs)具有激活初始T细胞、引发抗原特异性免疫反应的特性。本研究拟用体外培养的方法,从肺癌患者恶性胸腔积液中诱导出功能健全的DCs。方法:取16例肺癌患者胸腔积液500～1000ml,用密度梯度离心辅以免疫磁珠分选的方法,分离出DCs前体细胞,加入IL鄄4、GM鄄CSF、TNF鄄α诱导出DCs。用电镜和光镜观察培养的DCs,用流式细胞仪检测DCs的表面分子。将不同培养时间的DCs和肿瘤浸润性淋巴细胞(tumorinfiltrativelymphocytes,TILs)作混合淋巴细胞反应,了解DCs对TILs的激活作用。结果:在体外能诱导出恶性胸腔积液来源的功能健全的DCs,电镜和光镜分析表明,这类DCs具有成熟DCs的典型形态;当DCs体外培养到第48h时,表面分子的表达率与培养0h、24h、92h、192h的DCs比较相对较高;DCs可使TILs细胞扩增。结论:可从肺癌患者恶性胸腔积液中诱导具有成熟功能的DCs。
BACKGROUND & OBJECTIVE: Dendritic cells (DCs) can activate immunologic naive T cells to initiate antigen-specific immune responses. This study was to in vitro induce mature DCs from malignant pleural effusions of patients with lung cancer. METHODS: Malignant pleural effusions (500-1 000 ml) were collected from 16 patients with primary lung cancer. Precursory DCs were obtained through density gradient centrifugation and magnetic cell sorting from malignant pleural effusions, and cultured with interleukin-4 (IL-4), granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α). Morphology of DCs was observed under invert optical microscope and electronic microscope; phenotype of DCs was analyzed by flow cytometry. Effect of DCs on proliferation of tumor infiltrative lymphocytes (TILs) was observed through mixed lymphocyte reaction (MLR). RESULTS: Mature DCs with typical morphology (elongated dendritic processions observed under invert optical microscope and electronic microscope) were induced from malignant pleural effusions. Expressions of surface phenotypes were higher in DCs induced for 48 h than in DCs induced for 0, 24, 96, and 192 h. When activated with DCs, proliferation of TILs was enhanced. CONCLUSION: Mature DCs could be induced from malignant pleural effusions of patients with lung cancer.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2005年第6期663-666,共4页
Chinese Journal of Cancer
基金
高等学校骨干教师资助计划(No.2000-65)
广东省第十三批重点科技项目(No.2000-261-26)
广东省医学科研基金项目(No.A2000298)~~
关键词
树突细胞
肺肿瘤
恶性胸腔积液
肿瘤浸润性淋巴细胞
Dendritic cells
Lung neoplasms
Malignant pleural effusions
Tumor infiltrative lymphocytes
