摘要
目的探讨调节活化正常T细胞表达和趋化因子RANTES在腹主动脉瘤(AAA)形成中的作用。方法将Wistar大鼠56只随机分成7组,灌注法建立AAA模型。A组不灌注。E、F、G组腹腔注射吡咯烷二硫代氨基甲酸酯(PDTC)。分别在术后4、7、14d取标本。用苏木素伊红(HE)及免疫组织化学染色观察动脉壁炎性细胞浸润及RANTES的表达,并用逆转录聚合酶链反应(RTPCR)观察RANTESmRNA的表达。结果术后7d,RANTES及RANTESmRNA的表达最高,分别为(44.73±6.81)%和(81.58±5.73)%。术后14d形成AAA,动脉扩张率为(113.73±5.75)%。在E、F、G组,RANTES的表达受到抑制(P<0.05),且术后14d没形成AAA。结论RANTES在AAA形成之前表达升高,促进了AAA的形成。
Objective To investigate the role of regulated upon activation,normal T cell expressed and secreted (RANTES) in the development of abdominal aortic aneurysm (AAA) in rats.Methods Fifty-six Wistar rats were divided into 7 groups randomly.Pig elastinase was perfused into the abdominal aorta to construct AAA animal models.The abdominal aorta was harvested without being perfused in group A.Twenty-four rats in groups E,F,and G were injected with pyrrolidine dithiocarbamate (PDTC).The abdominal aorta was harvested on the postoperative day 4,7,14 respectively.HE staining and immunohistochemical staining were carried out to detect the infiltration of inflammatory cells and the expression of RANTES.The mRNA expression of RANTES was measured by RT-PCR.Results The expression of RANTES and RANTES mRNA was increased significantly on day 7,reaching (44.73± 6.81)% and (81.58± 5.73)% respectively.Fourteen days after elastase perfusion,aortic diameter was increased to aneurysmal proportions [(113.73± 5.75)% increase, P< 0.05],with inflammatory cells infiltrating and elastic fiber disrupting.In groups with injection of PDTC,the expression of RANTES and mRNA was inhibited (P< 0.05).Aortic diameter of these rats was not increased to aneurysmal proportions.Conclusion Increased rat aortic wall expression of RANTES occurs in the early development of AAA and RANTES accelerates the development of AAA.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第6期671-673,i001,共4页
Chinese Journal of Experimental Surgery
