摘要
目的:研究肺保护性通气策略联合山莨宕碱在急性肺损伤(ALI)动物模型中的肺保护作用。方法:将40只雌性wistar大白鼠经尾静脉注射油酸制成急性肺损伤模型,随机分成5组(每组8只):A组为对照组;B组为山莨宕碱治疗组(5mg·kg-1·h-1);C组采用大潮气量(15ml·kg-1)进行机械通气;D组采用小潮气量(5ml·kg-1)通气;E组为小潮气量机械通气联合山莨宕碱。分别监测各组治疗前后各时间点的动脉血气和mPAP;治疗4h后,处死大白鼠,取出肺组织,制片后在光镜下观察肺损伤程度。结果:与治疗前相比,A组氧合指数(OI)进行性下降(P<0.05);B组OI呈上升趋势(第3,4h点上升较为明显);D,E两组OI各时点也呈上升趋势,且各时点有显著性差异;C组OI呈先上升(1,2h明显高于其他组)后大幅度下降(在3,4h显著低于其他组)的趋势;E组在3,4h显著高于其他组。A组mPAP呈上升趋势,各时点均显著高于治疗前;B,D,E组呈下降趋势,在2,3,4h点均低于A组;C组前两时点显著低于治疗前和其他各组。C组的肺损伤积分均高于其他组(P(0.05),E组最低,B和D组得的肺损伤积分显著低于A组。结论:保护性肺通气策略联合山莨宕碱对实验性ALI动物模型具有协同治疗效果。
Objective: To investigate the efficacy of lung protective ventilation strategies combined with anisodamine in animal model of acute lung injury. Method: 40 rats'models of acute lung injury induced by oleic acid by injection via tail vein were randomly divided into five groups ( 8 rats each group ) after being anesthetized: controlled group (group A) , treated with anisodamine (5 mg/ (kg·h) , group B) ; ventilation with larger tidal volume (15 ml·kg-1 , group C) ; ventilatied with smaller tidal volume (5 ml·kg-1 , group D) ; ventilation with smaller tidal volume combined with anisodamine (group E). Then the arterial blood gas and the mean pulmonary arterial pressure ( mPAP) at different time points of pretreatment and posttreatment in each group were measured. The rats were executed four hours treatment, the lung tissues were taken out and the extents of lung lesion were observed under the microscope after section. Result: Compared with pretreatment, oxygenation index(OI) in rats of group A were declined (P < 0. 05) ; OI in rats of group B presented the trend of increase (more significant at 3 and 4 time point) ; OI in rats of group D and E rised also, and there was a obvious discrepancy among different time points; OI in group C indicated the trend of increase apparently at former two time points and that of drop remarkably at later two time points; OI in rats of group E were higher than other groups at 3 and 4 time points. mPAP in group A showed the increasing tendency, of which, each time points were obviously higher than that of pretreatment ; mPAP in group B, D, E presented declining tendency, of which ,2,3,4 time points were lower than that of group A; MPAP in group C decreased more sharply at previous two hours than that of all other groups (P <0. 05) . Ultimately , the lung injury score in group C was the highest among five groups ( P < 0. 05 ) , that of group E was the lowest ; compared with group A , the lung injury score in group B and D were lower. Conclusion: The lung protective ventilation combine
出处
《中国药师》
CAS
2005年第6期452-455,共4页
China Pharmacist
关键词
保护性通气策略
山莨菪碱
肺损伤
急性
Protective ventilation strategies
Anisodamine
Acute lung injury
