摘要
采用实验室模拟方法,以鲫鱼(Carassiusauratus)作为实验动物,运用SDS—PAGE和WesternBlotting方法,研究了在不同暴露时期,1mg/L2-硝基-4’-羟基二苯胺对鲫鱼肝脏和脑组织应激蛋白HSF70诱导表达的情况.结果表明:应激蛋白HSP70对2-硝基-4’-羟基二苯胺非常敏感.在鲫鱼暴露2d后,脑组织中HSP70与对照组相比,有显著的表达(p<0.05);在鱼体暴露7d后,肝脏组织中HSPT0与对照组有显著的诱导表达(p<0.05);随着暴露天数的增加,肝脏组织和脑组织HSP70表达也显著增加,且存在着较好的时间-效应关系.可考虑将应激蛋白HSP70作为2-硝基-4’-羟基二苯胺污染在水生生态系统中的一种敏感的生物标志物.
Carassius auratus was chosen as the experimental materials to study the dynamic effects of 1 mg/L 2-nitro-4' -hydroxydi-phenylamine on the induction of HSP70 in the fish livers and brains. Using the SDS-PAGE and Western Blotting techniques, the expression level of HSP70 in the fish livers and brains was evaluated. As shown from the results, 2-nitro-4' -hydroxydiphenylamine treatment resulted in a time-dependent elevation in HSP70 expression at 2, 4, 7 , 10 and 16 days exposure. Comparing with the control group,the HSP70 in the fish brains and livers began to express significantly after being exposed for 4 days and 7 days respectively. The expression level of HSP70 was enhanced with the exposure days increasing and showed a good time-effect relationship. Results from this study suggest that HSP70 as an environmental biomarker can be a sensitive and rapid indicator in the aquatic ecosystem.
出处
《湖泊科学》
EI
CAS
CSCD
北大核心
2005年第2期188-192,共5页
Journal of Lake Sciences
基金
国家自然科学基金重点项目(No:20237010)资助
