摘要
本实验用免疫组织化学PAP法和免疫金银法－PAP法免疫双重标记及常规电镜，对乌鳢A、B、D细胞的形态结构及分布进行了观察。用A蛋白盼体金（PAG）免疫电镜技术，对胰岛素进行细胞内精确的定位。同时，根据体视学有关立体重建的原理，从A、B细胞不同大小的切面的频数分布，重建了其球体大小的额数分布。
The distribution and morphology of the A,B and D cells in ophiocephalus Argus pancreas were observed with immunhischemistry (PAP method, PAP - protein A goldsilver double stalning) and electron microscopy. By PA-gold(PAG) method,the prescise intracgllular localication of insulin was observed. The stereological algorithme for the conversion from the profile-size frequency distribution observed in histological section to the sphere-size frequency distribution in three-dimension space was developed to a computer program and were applied to A,B cells in the pancreas.
出处
《遵义医学院学报》
1994年第4期276-278,共3页
Journal of Zunyi Medical University
关键词
免疫组织化学
鱼鳢
胰腺
内分泌细胞
形态学
Immunohistochemisty
PA-gold method,the A, B, D cells of islet
stereological reconstruction
