摘要
目的研究生长抑素类似物奥曲肽诱导人肝癌细胞株细胞凋亡的作用,观察不同的人肝癌细胞株生长抑素受体(SSTR)亚型的表达情况。探讨SSTR亚型与肝癌细胞凋亡之间的关系。方法分别培养人肝癌细胞株(SMMC-7721,HHC-98,HepG2),荧光染色、透射电镜和流式细胞仪检测细胞凋亡;RT-PCR检测细胞SSTR亚型mRNA的表达。结果0.25-4.0μg/mL的奥曲肽作用48h后,三种肝癌细胞部分呈现典型的凋亡形态学改变,细胞凋亡率呈浓度依赖性升高。3种肝癌细胞株均表达SSTR2、3、4,SSTR5则在两种肝癌细胞株SMMC-7721和HHC-98中表达,所有细胞株均未检测到SSTR1的表达。结论奥曲肽可以诱导肝癌细胞凋亡,其作用与肝癌细胞中广泛表达的SSTR亚型相关。
Objective To investigate the effect of octreotide on apoptosis of different human hepatocellular carcinoma (HCC) cells and the expression of somatostatin receptor (SSTR) subtypes on HCC cells. Methods Three different kinds of hepatocellular carcinoma cells (HepG2, SMMC-7721 and HHC-98) were cultured in vitro, The apoptosis was detected by fluorescent staining, transmission electron microscope and flow cytometry, and SSTR subtype mRNAs of these cells were determined by reverse transcription polymerase chain reaction. Results With a few of octreotide concentrations (0. 25 μg/mL, 1. 0 μg/mL, 2. 0 μg/mL and 4. 0μg/mL ) were increased, apoptosis was induced by octreotide in HCC cells. SSTR2.3 and 4 were positive in all three hepatocellular carcinoma cells, and SSTR5 was found in SMMC-7721 and HHC-98 cells, no SSTR1 was detected in HCC cells. Conclusion SSTRs are widely expressed in hepatocellular carcinoma cells and the apoptosis induced by octreotide may mediated by SSTRs.
出处
《肿瘤》
CAS
CSCD
北大核心
2005年第3期232-235,共4页
Tumor
关键词
肝肿瘤
癌
肝细胞
细胞系
肿瘤
受体
生长抑素
细胞凋亡
Liver neoplasms
Carcinma.hepatocellular
Cell line,tumer
Recepors,somatostatin
Apoptosis
