摘要
目的研究中国汉族人群基质金属蛋白酶9(maxtrixmetalloproteinase9,MMP9)基因C1562T多态性与急性冠状动脉综合征(acutecoronarysyndrome,ACS)发病的关联性。方法用聚合酶链反应限制性片段长度多态性分析法分析101例经冠状动脉(冠脉)造影确诊的ACS患者MMP9基因的C1562T多态性,以同期冠脉造影阴性、排除冠心病诊断的105例患者为对照组,比较两组间MMP9基因多态性频率分布的差异,并结合造影情况,探讨MMP9基因多态性与ACS发病及冠状动脉狭窄程度的关系。结果ACS患者CT+TT基因型频率(27.7%)明显高于对照组(13.3%),两组差异有统计学意义(χ2=6.567,P=0.01),T等位基因频率在ACS组和对照组分别为14.9%、7.2%(χ2=5.617,P=0.018);MMP9基因C1562T多态性分布与ACS冠脉狭窄程度差异无统计学意义(χ2=0.601,P=0.896)。结论MMP9基因C1562T多态性可能与中国汉族人群ACS有关,MMP9基因1562T等位基因可能是ACS遗传易感性的基因标记之一;MMP9基因C1562T多态性与ACS冠脉狭窄程度无关。
Objective To investigate the association betwee n acute coronary syndrome(ACS) and functional matrix metalloproteinase-9 polymor phism(C-1562-T). Methods This study was conducted with a case -control design including 101 patients with angiographically documented ACS and 105 control subjects who were free from coronary artery disease and had norma l angiograms. Genotype was determined by polymerase chain reaction-restriction fragment lengt h polymorphism for the common C-1562-T functional promoter polymor phism of the MMP-9 gene.The relationship between the polymorphism i n the MMP-9 gene and the severity of coronary arterial stenosis was analyzed. Resu lts The results of individual polymorphisms analysis showed that the fr equency of C/T and T/T genotypes of the C-1562-T polyporphism(27.2%) in patien ts with ACS was significantly higher than that in those with a normal angiogram( 13.34%).The frequencies of -1562T allele were 14.9% and 7.2% in ACS group and c ontrol group respectively (χ~2=5.617,P=0018). The frequencies of C/T and T/T genotypes of the C-1562-T polymorphism were not statistically different am ong ACS patients with normal and one,two,three or more significantly diseased ve ssels(χ~2=0.601,P=0.896). Conclusion The present findi ng s suggest that the genetic polymorphism in MMP-9 promoter (C-1562 - T) is associated with the susceptibility to ACS in the Han population of China. And the C-1562-T polymorphsim may not be useful as a predictor of the severity of coronary atherosclerosis.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2005年第3期313-316,共4页
Chinese Journal of Medical Genetics
