摘要
目的明确小肠移植排斥反应中的细胞凋亡现象,并探讨白细胞介素1β(IL1β)在凋亡中的作用。方法选用SD/Wistar大鼠进行节段性小肠移植。实验分3组:同基因移植组(SD→SD);异基因移植组(Wistar→SD)和异基因移植加环孢素A治疗组(Wistar→SD+CsA)。术后第1,3,5,7天分别用TUNEL法检查移植肠上皮凋亡细胞,用ELISA法测定血清IL1β。结果TUNEL法显示:Wistar→SD组肠黏膜上皮细胞在其发生轻、中、重度排斥反应中存在细胞凋亡,凋亡细胞数随排斥反应的加重而增加(P<0.01),并显著高于SD→SD组(P<0.01)。Wistar→SD+CsA组细胞凋亡数亦有显著增加(P<0.01)。SD→SD组排斥反应轻微,凋亡细胞数无明显变化(P>0.05)。ELISA法显示:在SDSD组血清IL1β无明显变化(P>0.05),WistarSD组和Wister→SD+CsAz组随凋亡细胞数的增加血清IL1β亦增高(P<0.01)。IL1β增加与细胞凋亡指数增加呈正相关(r=0.798,P<0.01)。结论小肠移植排斥反应中存在细胞凋亡现象,IL1β在细胞凋亡发生中起促进作用。
Objective To study apoptosis of small bowel epithelial cells during rejection of small bowel grafts and explore the correlation between apoptosis and cytokine(Interleukin-1β). Methods Heterotopic small (intestinal) transplantation was performed in SD and Wistar rats. All recipients were (divided) into three groups: Group I isograft(SD-SD);Group II allograft(Wistar-SD);Group III treatment control(Wistar -SD treated with cyclosporine A). The grafts were harvested on day 1, 3, 5, 7 after operation. All graft samples were examined for apoptotic cells of graft epithelial cells with TUNEL method. Serum IL-1β levels were (detected) by sandwich ELISA method. Results The TUNEL method showed that in Wistar→SD group, (apoptosis) of (intestinal) mucosa epithelial cells occurred during mild, moderate and severe rejection, and the number of (apoptotic) cells increased with increase of severity of rejection(P<0.01), and was significantly higher than in SD→SD group(P<0.01). The number of apoptotic cells was also increased in Wistar→SD+CsA group(P<0.01). Rejection was mild in SD→SD group, and the number of apoptotic cells was not (significantly) changed(P>0.05). ELISA method showed no significant change of serum IL-1β in SD→SD group(P>0.05), but in Wistar→SD group and Wistar→SD+CsA group, the serum IL-1β (levels) (increased) as the number of apoptotic cells increased(P<0.01). Increase in level of IL-1β and (increase) in number of apoptotic cells showed a significant positive correlation(r=0.798, P<0.01). Conclusions Apototic phenomena are present during rejection of small intestinal graft. IL-1β can promote the (development) of cell apoptosis.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第6期448-450,共3页
China Journal of General Surgery
关键词
肠
移植
移植物排斥
肠黏膜
细胞凋亡
白细胞介素1Β
Intestines/transpl
Allograft Rejection
Intertones Mucosa
Apoptosis
Interleukin-1β
