摘要
胆碱磷酸转移酶基因(cpt)是磷脂合成代谢途径上一个关键的酶基因,其基因产物是磷脂酰胆碱(PC),PC做为主要膜脂与膜的流动性和植物的抗寒性有密切关系。本实验用花粉管通道法将质粒PRDH401(含35S-35S-AMV-cpt-NOST)导入五个黑龙江常见栽培大豆品种中,对D1 代植株进行卡那霉素抗性筛选、低温筛选和PCR检测,初步筛选到转化植株。
Cold resistance in the low temperature-induced plants is closely related to the synthesis metabolism path of phospholipids.Cholinephospho transferase gene is a key enzyme gene in the synthesis metabolism path of phospholipids.Phosphotidy choline(PC)is the enzyme gene product of CPT gene and it is the key component of the membrane..It is closely related with the mobility of membrane and cold resistance. In this experiment,plasmid PRDH401 which carrying the 35S-35S-AMV-cpt-NOST fusion gene,was transferred into 5 varieties of soybean by means of the pollen tube pathwa.We got the D-1 generation plants in the next year.Through the selection of kanamycin and low temperature,several transformed seedlings were obtained.PCR of the transformed soybean DNA indicated the integration of cpt gene into the transgenic soybean genome.
出处
《大豆科学》
CAS
CSCD
北大核心
2005年第2期150-153,共4页
Soybean Science
基金
黑龙江省教育厅项目(10511031)
