摘要
目的探讨大体积培养人外周血淋巴细胞染色体标本技术在学生实验中的应用及意义。方法应用大体积培养液培养人外周血并制备染色体标本,与常规方法进行比较。结果实验组染色体标本良好率85.5%,一般率11.9%,较差2.6%。对照组标本良好率85.9%,一般率10.9%,较差占3.2%,两组标本质量无明显差异(P>0.05)。结论大体积培养人外周血淋巴细胞制备的染色体标本质量可靠,适用于大规模学生实验。
Objective To study the application and significance of making chromosome specim peripheral blood lymphocyte(PBL) by large volume culture for students experiment.Methods The PBL with large volume was cultured,the chromosome specimen was made,then it was compared with routine method.Results About the quality of chromosome specimen, in experimental group were well 85.5%, ordinary 11.9%,and worse 2.6%;In contrast group were well 85.9%,ordinary 10.9%,and worse 3.2%.The quality of the two group of chromosome specimen is no obvious difference(P>0.05).Conclusion The quality of chromosome specimen in PBL made by the new method is dependable.It is available to many students′ experiment.
出处
《新乡医学院学报》
CAS
2005年第3期228-228,230,共2页
Journal of Xinxiang Medical University
关键词
大体积培养
人外周血淋巴细胞
染色体标本
large volume culture
human peripheral blood lymphocyte
chromosome specimen
