摘要
目的研究不同组织分离方法、肿瘤TNM分期、细胞分化程度等因素对喉癌组织原代培养细胞生长率的影响。方法对17例喉癌组织分别应用组织块、胶原酶和机械分离法进行原代培养,观察以上3种组织分离法、肿瘤细胞分化程度以及TNM分期对喉癌原代培养细胞生长情况的影响,并用统计学分析其结果。结果组织块、胶原酶及机械分离法的细胞生长率分别为64.7%、58.8%和11.8%,其中组织块法和胶原酶法比较差异无统计学意义(P=1.000),而织块法和胶原酶法与机械分离法比较差异均有统计学意义(P=0.004和0.008)。鳞癌细胞高分化组细胞生长率为33.3%,中/低分化组为90.9%,2组比较差异有统计学意义(P=0.028)。结论细胞培养的生长率与肿瘤组织的分化程度和组织分离方法有关。在喉癌的原代培养中应尽量选取细胞分化程度差的标本并且选用组织块分离法进行培养。
Objective To study the factors which affect the growth rate of the primary culture of the laryngeal squmous cell carcinoma. Methods Seventeen specimens of resected laryngeal squamous cell carcinoma tissues were obtained for primary cell culture and each of them was dissected in three pieces. Three pieces were dissociated by shearing, collagenase and physical methods, respectively. The results of these three methods and other factors were analyzed by χ~2 test. Results Cell growth rate were 64.7% in shearing group, 58.8% in collagenase group and 11.8% in physical group. The growth rate between the shearing group and collagenase group had no significant difference (P=1.000) while that of the physical group was much less than the other two groups (P=0.004, P=0.008). The tumor differentiation affected the growth rate. For well differentiated tumors the growth were 33.3% and for mildly/poorly differentiated ones the rate were 90.9%(P=0.028). In the study, there was no correlation between the TNM stage and the growth rate. Conclusion The growth rate is higher with tumors from patients with poor differentiated cells. The shearing method has high cell growth rate and less procedure, so it is the best method.
出处
《首都医科大学学报》
CAS
2005年第3期242-245,共4页
Journal of Capital Medical University
基金
国家自然科学基金(30070811)资助项目
