摘要
采用斑点杂交法检测了重庆地区263份2～3月龄麻鸭血清中的DHBV DNA,用筛选出的阳性病毒血清经腹腔感染1～2日龄的血清DHBV DNA阴性雏鸭,并用该模型进行了21种中药抗乙肝病毒初(?)。结果表明:(1)重庆地区2～3日龄麻鸭DHBV自然感染率为25.48％.(2)经腹腔感染的1～2日龄雏鸭的血清DHBV DNA阳性率1周后为82.90％,并可持续至少2个月。(3)抗乙肝病毒中药初(?)发现用广西叶下珠、苦参治疗2周可使DHBV DNA滴度下降,其余的经体外实验认为有效的抗乙肝病毒中药,动物实验并未获证实。
Uninfected 1-2 day-old Chongqing ducklings were inoculated intraperioneally with serum containning DHBV to establish duck hepatitis B animal model. We used the model for screening 21 Kinds of tradional Chinese medicine. The following results were obstained: (1) The natural infected rate of 2-3month-old ducks is 25. 48%. (2) These ducklings inoculating with DHBV can produced a persistent infection over 2 month and infected rate was 82.90%. (3) The serum DHBV DNA was decreased after the treatment 2 weeks with Guangxi Phyllanthus, Ku Shen. No significant effect was observed in other drugs in the animal test.
出处
《中西医结合肝病杂志》
CAS
1994年第3期25-27,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
