摘要
用硝基蓝四氮唑(NBT)试验观察大鼠重症烧伤后血内中性粒细胞(PMNs)激活情况与动物存活时间的关系。结果表明,烧伤后总的 PMNs 激活率比对照组明显增高,伤后5 h 激活程度与动物存活时间呈显著负相关。连续测定显示,烧伤后 PMNs 激活率无明显增高,或先升高后降至正常水平者存活时间长,预后较好。而 NBT 阳性率持续不断增高者,存活时间明显缩短,预后不良。提示 NBT 连续测定对判定重症烧伤预后有重要意义。此外,虎杖治疗重症烧伤后大鼠 NBT 阳性率降低,其存活时间比未治疗组明显延长。
Using NBT test,the relationship between the percentage change of acti- vated neutrophils with the survival time of animals after severe burns was observed. The results showed that the NBT positive rate of neutrophils increased markedly after burn,and the NBT positive rate at 5 h postburn was negatively related to the survival time of burned animals.Continuous monitoring of neutrophil indicated that if the NBT positive rate alter burn remained in the normal range,or increased initially to high level and then decreased to lower than normal,the survival time was longer.However,if the NBT positive rate increased continuously after burn,the survival time was shorter. Therefore,continuous assay of neutrophil activation with NBT is an important index for the prognosis of severe burns.Lowering of NBT positive rate was observed in rats treat- ed with polygonum cuspidatum,and these rats lived much longer than those without the treatment.
基金
国家自然科学基金
关键词
烧伤
中性粒细胞
预后
NBT
Burn
Neutrophil activation
Polygonum cuspidatum
