摘要
目的:探讨PrP10 6- 12 6对神经元的毒性作用。方法:原代培养的皮质神经元暴露于合成多肽PrP10 6 -12 6,观察细胞存活率的变化,采用流式细胞术检测有无凋亡的发生,用免疫印迹法检测caspase 3的活性。结果:在PrP10 6 -12 6的作用下,神经元存活数较对照组减少,随着作用时间的延长,存活率明显下降。在PrP10 6- 12 6作用下神经元的凋亡比例明显增加,PrP10 6- 12 6组凋亡细胞为2 4.93 % ,对照组为6.95 %。与对照组比,PrP10 6 -12 6组在培养第6、8、10天时均有较明显的caspase- 3表达,且伴有caspase 3的活化。结论:PrP10 6- 12 6对原代培养的皮质神经元有毒性作用,呈时间依赖性。PrP10 6 -12 6毒性作用涉及了细胞凋亡机制,激活caspase -3诱导细胞凋亡可能是途径之一。
Aim:To explore the neurotoxic effects of PrP106-126 on the primary cortical neuron culture. Methods: Primary cortical neuron cultures were treated with 80 μmol·L -1 PrP106-126 and the cell viability or death was determined by using the MTT assay. Flow cytometry was used for detecting the apoptosis of cells. The activity of caspase-3 was detected by Western blot. Results:The neuronal viability was significantly decreased in the group treated with PrP106-126. At the 6th day, the neuronal viability was decreased to approximate 50%, and the viability decrease was more evident with prolongation of time. The percent of apoptotic cell was 24.93% in the group of PrP106-126, but was 6.95% in the control group. Moreover, the caspase-3 was activated at the 6 th, 8 th and 10 th day in the group of PrP106-126. Conclusion:The neurotoxicity of PrP106-126 to the primary neuron culture acts in a temporally dependent manner. Apoptosis occurs in primary neuron culture. The activation of caspase-3 pathway is involved in the apoptosis induced by PrP106-126.
出处
《中国临床神经科学》
2005年第2期140-143,共4页
Chinese Journal of Clinical Neurosciences
