摘要
目的:探讨胰淀素对去势雌性大鼠骨质疏松症的干预作用。方法:实验于2003-02/2004-05在解放军第三军医大学大坪医院野战外科研究所妇产科实验室完成。选用3月龄Wistar大鼠40只,随机分为3组:正常组(n=8)、去势组(n=8)、胰淀素治疗组(n=24)。胰淀素治疗组按治疗剂量分为3组:10μg/kg组(n=8),50μg/kg组(n=8)和250μg/kg组(n=8)。正常组不作任何处理,不造成骨质疏松模型;去势组予以同量生理盐水注射;胰淀素治疗组给予胰淀素相应剂量皮下注射,1次/d。16周后行骨密度、骨生物力学及血、尿生化检查。结果:40只大鼠均完成实验。①大鼠腰椎和股骨骨密度:去势组均明显低于正常组(t=3.125,3.367,P<0.01),胰淀素治疗组明显高于去势组(t=2.895～4.589,P<0.01)。其中以50和250μg/kg胰淀素干预治疗后骨密度接近于正常组。②尿钙、尿磷、尿羟脯氨酸值:胰淀素治疗组明显低于去势组(t=2.578～4.487,P<0.01)。③血骨钙素:正常组明显高于去势组犤(1.33±0.25),(0.88±0.18)μg/L,P<0.01犦;胰淀素治疗组明显高于去势组犤(0.97±0.17),(1.18±0.21),(1.24±0.22)μg/L;(0.88±0.18)μg/L,t=2.698,3.358,4.438,P<0.01犦。④股骨最大负荷、最大应变、最大应力变化:胰淀素治疗组明显高于去势组(t=2.958～4.258,P<0.01),250μg/kg胰淀素治疗组大鼠股骨力学指标接近正常组。⑤血清及尿生化指标变化:以250μg/kg胰淀素治疗后最接近正常组。结论:胰淀素治疗可以增加去势大鼠骨密度,提高大鼠股骨生物力学性能。生化检查提示胰淀素不仅可以促进骨形成,同时还具有抑制骨吸收的作用,其改善血尿生化指标及骨生物力学性能以250μg/kg胰淀素干预效果最好。
AIM:To investigate the interventional effect of amylin on osteoporosis in female ovariotomized rats.METHODS:The experiment was carried out in the laboratory of Department of Obstetrics and Gynecology,Research Institute of Surgery,Daping Hospital,Third Military Medical University of Chinese PLA between February 2003 and May 2004.Forty 3 month old Wistar rats were randomly divided into 3 groups:normal group(n=8),ovariotomized group(n=8) and amylin treated group(n=24),and the amylin group was subdivided into 10 μ g/kg group(n=8),50 μ g/kg group(n=8) and 250 μ g/kg group(n=8).Rats in the normal group received no treatment,and were not made into models of osteoporosis;Rats in the ovariomotized group were injected with saline of the same volume;Rats in the amylin treated groups received subcutaneous injection of amylin of corresponding dosages, once a day.After 16 weeks,bone mineral density,bone biomechanics, and blood and uric biochemical examinations were detected.RESULTS:All the 40 rats finished the experiment.① The bone mineral densities at lumbar spine and femur were obviously lower in the ovariotomized group than in the normal group(t=3.125,3.367,P< 0.01),markedly higher in the amylin treated group than in the ovariotomized group(t=2.895 to 4.589,P< 0.01);After treatment,the bone mineral densities in the 50 and 250 μ g/kg amylin groups were close to those in the normal group.② The values of calcium, phosphorus and hydroxyproline in urine were obviously lower in the amylin treated groups than in the ovariotomized group(t=2.578 to 4.487,P< 0.01).③ Blood osteocalcin level was obviously higher in the normal group than in the ovariotomized group[(1.33± 0.25),(0.88± 0.18) μ g /L,P< 0.01],markedly lower in the amylin treated groups than in the ovariotomized group[(0.97± 0.17),(1.18± 0.21),(1.24± 0.22) μ g /L,(0.88± 0.18) μ g /L,t=2.698,3.358,4.438,P< 0.01].④ The maximal load, the maximal strain and the change of the maximal strain were obviously higher in the amylin treated groups than in the ovario
出处
《中国临床康复》
CSCD
北大核心
2005年第19期172-173,共2页
Chinese Journal of Clinical Rehabilitation
