摘要
目的:探讨在常氧、缺氧状态下,豚鼠心肌细胞内游离钙离子浓度与葛根素注射液干预的影响,并与硝苯地平的作用相比较。方法:采用离体豚鼠心脏Langendorff法灌注,I型胶原酶分离心肌细胞,并调整细胞数为2×109L-1。在细胞外钙含量为2mmol/L情况下,采用F-2000型荧光分光光度计进行心肌细胞内钙含量测定,计算细胞内游钙离子浓度。将心肌细胞悬液分为6组,分别为常氧及缺氧条件下的对照组、葛根素注射液组、硝苯地平组。在常氧条件下(氧分压=15.9kPa),观察100s后,随机选择3组分别加入营养液10μL、葛根素注射液10μL(终浓度:0.1mmol/L)、硝苯地平l0μL(终浓度:20mmol/L)观察100,200,300,400,500s。而另外3组在缺氧条件下(氮气:0.1cm3/s)也分别给予同浓度的上述3种药物后,按上述5个时间点进行观察。采用荧光分光光度计动态测量不同条件下各组心肌细胞内游离钙离子浓度的变化。结果:①在静息(0～100s)常氧状态(氧分压=15.9kPa)及控制细胞外细胞内游离钙离子浓度为2mmo1/L(生理情况)时,6组的心肌细胞内游离钙离子浓度无显著差异(P>0.05),故知6组的组间一致性较好,具有可比性。②心肌细胞内游离钙离子浓度:葛根素注射液组和硝苯地平组给药后100,200,300,400,500s均明显低于对照组(P<0.05～0.01)。但葛根素注射液组下降速度较硝苯地平组慢(P<0.05～0.01)。③心肌细胞内游离钙离子浓度:缺氧状态下,葛根素注射液组缺氧后100,200,300,400,500s增加较对照组缓慢(163.7±19.7),(224.2±20.7),(309.7±15.1),(424.7±14.0),(477.5±21.5)nmol/L;(208.8±28.9),(328.5±14.4),(401.8±21.2),(534.3±20.8),(751.4±36.0)nmol/L,P<0.01犦。硝苯地平组在缺氧后100,200,300,400,500s比对照组增加少(P<0.01),且降低速率较葛根素注射液组高犤硝本地平组:(109.4±19.6),(140.8±16.2),(203.1±15.2),(265.4±17.3),(301.7±24.1)nmol/L,P<0.01犦。结论:在常氧条件下葛根素注射液使心
AIM:To investigate the intracellular concentration of free calcium in the myocardial cells of guinea pig under the conditions of normal oxygen and hypoxia,study the effects of intervention of puerarin injection, and compare the effects with those of nifedipine.METHODS:The in vitro guinea pig hearts were perfused with Langendorff method,myocardial cells were isolated with collagenase(Type I),and the number of cells was adjusted to 2× 109 L- 1.Under the extracellular concentration of calcium was 2 mmol/L,the intracellular content of calcium in myocardial cells was determined with F 2000 type spectrofluorometer,and intracellular concentration of free calcium ion was calculated.The myocardial cell suspension was divided into 6 groups:control group,puerarin injection group,nifedipine group under normal oxygen and hypoxia respectively.Under normal oxygen(partial pressure of oxygen=15.9 kPa) after observation for 100 s,10 μ L nutrient solution, 10 μ L puerarin injection(terminal concentration:0.1 mmol/L) and 10 μ L nifedipine(terminal concentration:20 mmol/L) were given to 3 groups with random selection and observed for 100,200,300,400 and 500 s.The other 3 groups were treated with the above 3 drugs of the same concentrations respectively under hypoxia(nitrogen gas:0.1 cm3/s),and were observed at the above 5 time points.The changes of the intracellular concentration of free calcium ion in myocardial cells in each group under different conditions were dynamically measured with spectrofluorometer.RESULTS:① Under normal oxygen(partial pressure of oxygen=15.9 kPa) at rest(0 to 100 s) and the extracellular concentration of free calcium ion was controlled at 2 mmol/L(physiological condition),there were no significant differences in the intracellular concentration of free calcium ion in myocardial cells among the 6 groups(P >0.05),it was known that the concordance among the 6 groups was good, and they were comparable.② The intracellular concentration of free calcium ion in myocardial cells 100,200,300,400 and 500 s aft
出处
《中国临床康复》
CSCD
北大核心
2005年第19期142-144,共3页
Chinese Journal of Clinical Rehabilitation
