摘要
应用聚合酶链反应（ＰＣＲ）对１０３例冠心病人及１００名正常人载脂蛋白Ｂ（ａｐｏＢ）基因５＇端信号肽序列的多态性研究结果表明：（１）中国人信号肽序列插入／缺失（Ｉｎｓ／Ｄｅｌ）多态性频率分布与国外不同种族间有差异。少见Ｄｅｌ等位基因相对频率为０．２５９，高于南亚人，但低于法国高加索人。（２）冠心病组与正常对照组间等位基因相对频率分布差异无显著意义。（３）冠心病组内具Ｄｅｌ等位基因者血浆ＨＤＬ一Ｃ水平明显低于不具Ｄｅｌ等位基因者（Ｐ＜０．０ｌ），提示ａｐｏＢ信号肽序列的多态性可能对脂质代谢有一定影响，其机理不明。（４）ＰＣＲ方法是鉴别ａｐｏＢ信号肽序列多态性的可靠方法。
nsertion / deletion (Ins / Del) polymorphism ofthe apo B gene encoding
signal peptide and its relationto coronary heart disease (CHD) and lipid trait werestudied in 103
patients with CHD and 100 healthy in-dividuals from Chinese Han nationality. The frequen-cy of
rare Del allele was 0.259, which was significantlylower than 0.34 in Caucasians (France), but
higherthan 0.16 in South Asian descents in UK. When thefrequency distribution of the Ins/Del
alleles wascompared between CHD group and control group, thefrequency of rare Del allele in
the patient group wasnot apparently different from that in the control group(0.282 vs. 0.235, P>0.
05). As for the impact of theIns/Del genotype on levels of lipid andapolipoprotein, the patients
with at least one Del allelehad significantly lower HDL-C level compared withthose with only ins
allele in the CHD group (P < 0.0 1 ).Levels of other lipid parameters in each group werenot
obviously associated with the Ins / Del genotype.It is suggested that the Ins / Del polymorphism
of theapo B signal peptide might be involved in lipidmetabvolism by an unidentified mechanism
in someethnic population. PCR is a useful method for distin-guishing Ins / Del polymoophism of
the apo B signalpeptide.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第6期341-344,共4页
National Medical Journal of China
基金
卫生部"八.五"基金
关键词
聚合酶链反应
冠心病
载脂蛋白
Apolipoproteins Genefrequency Coronary disease
Polymerase chain re-action.
