摘要
Ｋｎｖ－Ⅰ是一种新近发现的抗癌基因，目前对其在白血病发病中的作用研究甚少。利用逆转录多聚酶链反应扩增Ｋｒｅｖ－ⅠｃＤＮＡ片段，然后重组入质粒中而制备出Ｋｒｅｖ－ⅠｃＤＮＡ探针，使用该探针进行Ｎｏｒｔｈｅｒｎ杂交检测Ｋｎｖ－ⅠｍＲＮＡ表达水平，与正常骨髓细胞相比较，发现白血病细胞系ＨＬ－６０、Ｕ－９３７中Ｋｒｅｖ－ⅠｍＲＮＡ表达水平明显降低；１４例急性髓系白血病中，１例表达与正常相当，２例轻度降低，９例明显降低，２例无Ｋｒｅｖ－ⅠｍＲＮＡ表达。说明Ｋｒｅｖ－Ⅰ表达水平的降低在白血病的发生中可能起着重要的作用。
s an antioncogene, Krev-Ⅰ gene shares homology toras oncogene especially in effector region. It inhibitstransformation by competing for effector with ras-p21.However, the role of Krev-Ⅰ gene in leukemogenesisremains unclear. The fragment of Krev-Ⅰ cDNAobtained by reverse transcription and polymerase chainreaction (RT-PCR)was inserted into plasmidpBluescript Ⅱ SK￣+. The probe was prepared from therecombinant plasmid and then hybridized to total RNAsfrom 14 cases of acute myeloid leukemia and HL60,U937 cell lines. Compared with normal bone marrowcells, the expression level of Krev-Ⅰ mRNA was normalin one, decreased slightly in two, and decreasedmarkedly in nine. In the other two patients, there wasno expression of Krev-Ⅰ mRNA. In both cell lines Krev-Ⅰ mRNA expression was also decreased markedly. Ourresults suggested that the low expression of Krev-ⅠmRNA might play an important role in leukemogenesis.
出处
《中华血液学杂志》
CSCD
北大核心
1994年第7期353-355,共3页
Chinese Journal of Hematology
关键词
抗癌基因
聚合酶链反应
白血病
Antioncogene Polymerase chainreaction Acute myeloid leukemia
