摘要
本文利用SDSPAGE电泳方法鉴定了2001～2003年参加省区试的18份小麦品系的高分子量麦谷蛋白亚基组成,并根据GluDly位点的基因序列设计的一对引物,通过PCR技术对这些小麦品系的基因组DNA进行扩增,可以准确鉴别亚基组成为2+12和5+10的不同小麦品系。同时,用不同亚基组成亲本的杂交后代进行扩增,可以从F2后代群体中选择含有5+10亚基的单株,为分子标记辅助选择在小麦品质育种中的应用提供了基础。
The composition of high molecular weight glutenin subunit (HMW-GS) of eighteen regional testing wheat lines in Guizhou province during 2001-2003 were identified with the method of SDS-PAGE electropherogram. With a pair of synthetic primers by specific variation for Glu-Dly, different wheat lines with subunit pair 2+12, 5+10 can be distinguished clearly by genome DNA being amplified with PCR approach. At the same time, amplifying the hybrids whose parents contained different subunits, the plants contained subunit pair 5+10 can be selected from F 2 generation .PCR approach provided a base on molecular marker-assisted selection in early generation of wheat breeding program.
出处
《种子》
CSCD
北大核心
2005年第6期7-10,共4页
Seed
基金
贵州省"十五"攻关项目(15001A9
(2001)1112)
贵州省自然科学基金项目(黔基合计字(2001)3032号)资助。
