摘要
本文采用生物发光法测定玉米籽粒中ATP含量,ATP在0 0 1～0 5 μg/mL的浓度范围内,最大发光强度与浓度的对数成线性关系,线性回归方程Y =0 9973+ 3 6 6 5 1,相关系数为0 9991;RSD值为1 72 7% ;检出限为7 80×10 - 5μg/mL ;平均回收率94 5 % ,灵敏度、精密度高,结果准确。
The contents of ATP in maize kernel were determined by bio-Luminescence.A lon-linear calibration graph which was y=0.9973x+3.6651(r=0 9991),was obtained in the range of 0.01μg/mL to 0.5 ug/ml with detection limit of 7.80×10 -5 μg.mL for ATP.The RSD value was 1 727% and the average recovery of ATP was found to be 94.5%.The method was sensitive and accurate,can be applied to determinate the contents of ATP in plant organs.
出处
《现代仪器》
2005年第3期34-35,共2页
Modern Instruments
