摘要
目的探讨电磁场对骨肉瘤细胞系MG63细胞化疗增敏作用的机制。方法以频率为50Hz,强度为0.8mT、1.6mT、3.2mT的电磁场,对具有一定阿霉素浓度的MG63细胞进行磁场干预,采用四甲基偶氮唑蓝(MTT)法检测并确定磁场对骨肉瘤化疗作用最明显的磁场强度,逆转录酶聚合酶链反应技术(RT-PCR)检测在最佳磁场作用下增殖细胞核抗原(PCNA)和SurvivinemRNA的表达以及流式细胞仪检测细胞周期情况。结果MTT显示磁场化疗组(D组)细胞生存率较单纯阿霉素化疗组(B组)下降,且1.6mT作用最明显;RT-PCR结果显示PCNAmRNA、SurvivinemRNA表达下降;流式细胞仪检测D组G2/M期阻滞较B组高,D组G2/M期细胞达(80.20±0.96)%,高于B组的(18.08±1.50)%、单纯磁场组(C组)的(55.11±2.20)%(P<0.05)。结论适当窗口的电磁场作用可以提高肿瘤的化疗效果,为临床化疗提供一个新的辅助措施。
Objective To investigate the sensitizing mechanism of electromagnetic fields (EF) on chemotherapy of osteosarcoma cell line MG63. Methods MG63 cell line, co-incubated with Adriamycin, was interfered by EF with intensities of 0.8 mT, 1.6 mT and 3.2 mT, respectively. The most obvious intensity of EF for the chemotherapy of osteosarcoma was determined by using MTT assay, while the expression of proliferation cell nuclear antigen (PCNA) mRNA and survivine mRNA in the most suitable EF was measured with reverse transcription polymerase chain reaction (RT-PCR), the cell cycle was observed by using flow cytometry. Results It is shown by MTT that the survival rate of cells in the chemotherapy plus EF group (group D) was significantly decreased as compared to that in the Adrianycin group (group B), especially at 1.6 mT. The expression of PCNA mRNA and survivine mRNA was decreased according to the results of RT-PCR. It is indicated by the results of flow cytometry that the amount of cells, obstructed in G_2/M phase, was increased in group D (80.2±0.96)% with comparison of that in group B (18.08±1.5)% and that in the EF group (group C) (55.11±2.2)% (P<0.05). Conclusion EF with appropriate intensity can enhance the effects of chemotherapy on tumor, and it can provide clinical chemotherapy with a new assistant way.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2005年第5期269-271,共3页
Chinese Journal of Physical Medicine and Rehabilitation
