摘要
本文报道用ＨＣＶＮＳ５区两段抗原性较好的合成肽研制的ＥＬＩＳＡ试剂盒及ＵＢＩＨＣＶＮＳ５区抗体检测ＥＬＩＳＡ试剂盒观察１４例ＨＣＶ感染者ＮＳ５区抗体的动态变化，证实ＮＳ５区抗体如同ＮＳ４区抗体一样比Ｃ及ＮＳ３区抗体出现晚。ＮＳ５区抗体总体检出率近似于ＮＳ４区抗体；１．５５％的血清为单独ＮＳ５区抗体阳性；存在ＮＳ５区抗体与其他区抗体滴度有互补作用的标本等提示ＮＳ５区抗体仍有一定的诊断价值。采用ＵＢＩＮＳ５区抗体检测试剂盒发现，９５．６５％（２２／２３）ＵＢＩ试剂盒诊断为ＮＳ５区抗体阳性的标本中含ＨＣＶＲＮＡ，６／１４ＨＣｖ感染者用ＵＢＩ试剂盒检出的抗体出现在ＡＬＴ再次异常升高或剧烈升高（高于参考值的３倍以上）前后，４／１４的感染者抗体出现于ＡＬＴ首次升高前后（其余４／１４的感染者未检出抗ＮＳ５抗体），因此ＵＢＩ抗ＨＣＶＮＳ５抗体诊断试剂盒检测的抗体似与疾病的活动有关。
The dynamics of antibody to HCV NS 5 was observed with ELISA coated with 2
different peptides and UBIanti-HCV NS5 antibody diagnostic kit ,respectively. It confirmed that
anti-HCV NS5 antibody appeared later thanthe antibodies to HCV core and NS3 region. 1. 55%of
sera were found to contain antibodies only to NS5 region. The efficiency of ELISA for HCV
antibodies could be improved by adding NS5 antigen as coated antigen. It alsosuggests that
antibody detected with UBI HCV NS5 antibody diagnostic kit may have a good correlation with
theactivity of hepatitis,
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第2期73-76,共4页
Chinese Journal of Microbiology and Immunology
关键词
输血后
丙型肝炎病毒
ELISA
试剂盒
Post-transfusion hepatitis
ELISA kit
Antibody to HCV NS5
region
