摘要
对一组临床应用的全血（配血用标本）、血浆制剂与丙种球蛋白制剂进行检测。血浆制剂抗ＨＣＶ阳性率显著高于全血（３２．６４％与１１．６８％、Ｐ＜０．０１）。三组标本中抗ＨＣＶ阳性者ＨＣＶＲＮＡ检出率基本相似。但丙球制剂组ＨＣＶＲＮＡ阳性标本在ＰＣＲ中的反应强度显著低于前两者。对部份标本作ＨＣＶ基因亚型检测，三组总体ＨＣＶ亚型表达频率为，原型：２．９４％；Ｋ１：４７．０６％；Ｋ２ａ：３２．３５％；Ｋ２ｂ：１４．７１％及Ｋ３：２．９４％等，与文献中有关我国ＨＣＶ基因亚型的报道类似。基因亚型检测全血组以Ｋ１及Ｋ２型为主（３８．４１％及３０．７７％）、混合型亦占相当比例（２０％）。血浆组ＨＣＶ亚型分布与全血组相近，但混合型占４７．６７％，这与该制剂系由多份献血员血浆混合制备有关。而同为多份血混合，经分层提取的丙种球蛋白制剂ＨＣＶ亚型以Ｋ２ａ为主（占７５％），未检出混合型。
e have detected anti-HCV,HCV RNA and HCV RNA subtypes of whole blood
,fresh human plasma and r-globulin all of which were from rural blood donors in Wuhan
area.The results showed that the detection rate of an-ti-HCV in pooled plasma was higher than
that of whole blood group (32. 46%and 11.68%,P<0.01).The resultsof HCV RNA detection rates in
all anti-HCV positive samples in the three groups were similar ,but a weaker reac-tion in PCR
assay had been shown in the r-globulin group.For detecting HCV RNA subtypes,the rates of
variousHCV genetic subtypes were2.94%in HCV Pt,47.06%in HCV K1,32.35%in HCV K2a,14.71%
in HCV K2band2.94%in HCV K3.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第4期246-248,共3页
Chinese Journal of Microbiology and Immunology
基金
国家科委"八
五"攻关计划资助
关键词
丙型肝炎
全血
血液制品
基因分型
Hepatitis C
Anti-HCV
HCV RNA
HCV RNA subtype
PCR.
