摘要
构建了一个含人乳头瘤病毒１６型Ｅ６Ｅ７反意基因片断的逆转录病毒载体ｐＨ１９。用Ｌｉｐｏｆｅｃｔｉｎ将ｐＨ１９导入病毒包装细胞ｐＡ３１７，使之产生假型逆转录病毒Ｈ１９。Ｈ１９病毒感染人乳头瘤病毒１６型转化的细胞后，使转化细胞的生长速率和裸鼠体内形成肿瘤的能力均明显下降。
An amphitropic pseudotype retrovirus containing human papillomavirus E6E7 antisense sequences was constructed and packaged with helper cells (pA317). The recombinant virus infected cells which were transformed by HPV16 E6E7 ORFs showed that the growth rate of transformed cells decreased and the ability of forming tumor by these cells in nude mice was inhibited as compared with control. Thus, it appeared that HPV16-E6E7 antisense sequences mediated by retrovirus vector is capable of inhibiting cells proliferation and malignancy.
出处
《中华实验和临床病毒学杂志》
CSCD
1994年第1期16-19,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
逆转录病毒
假型
乳头状瘤病毒
Retrovirus vector
Human papillomavirus type 16
Pseudotype retrovirus
