摘要
经缺口平移法以ａ－３２Ｐ－ｄＣＴＰ标记１．０ｋｂ的丁型肝炎病毒（ＨＤＶ）ｃＤＮＡ片段为探针，采用蛋白酶Ｋ直接从血清中提取ＨＤＶＲＮＡ，建立了检测血清中ＨＤＶＲＮＡ的打点杂交法，其灵敏性可达１ｐｇ水平，与乙型肝炎病毒（ＨＢＶ）ＤＮＡ无交叉杂交反应；并应用于检测我国５９４９份ＨＢｓＡｇ阳性血清中的ＨＤＶＲＮＡ，共检出１７６份ＨＤＶＲＮＡ阳性，检出率为２．９５％。
A method for detecting HDV-RNA in serum samples has been developed by dot hybridization cDNA-RNA with 32p-labelled 1kb HDV cDNA probe. 1pg HDV cDNA was detectable and there was no cross-hybridization with HBV DNA. Five-thousand nine-hundred and forty-nine serum samples positive for HBsAg were tested by this assay and HDV-RNA was detected in 2.9% of these samples. This assay is a sensitive method to measure HD Viremia.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1994年第1期65-67,共3页
Chinese Journal of Experimental and Clinical Virology
