摘要
目的研究膀胱癌细胞系BIU-87体外转染pCH510后,CH50多肽的表达及其对卡介苗(bacilluscalmette-guerin,BCG)抑瘤作用的影响。方法在LipofectimineTM2000的介导下,将质粒pCH510体外转染给BIU-87细胞,采用免疫组织化学S-P法和WesternBlot法鉴定CH50多肽的表达;通过四唑盐比色法(MTT法)观察pCH510转染BIU-87对BCG细胞毒作用的影响。结果BIU-87细胞转染pCH510后阳性表达CH50多肽,对照组则不表达;CH50多肽阳性表达增强了BCG对BIU-87的细胞毒性作用(P<0.05)。结论转染pCH510后,BIU-87细胞表达CH50多肽,并因此促进了BCG对膀胱癌细胞的抑制作用。
Objective To investigate the expression and identification of CH50 polypeptide in bladder tumor cell line BIU-87 by gene transfection of pCH510,and study the effect on the antitumor function of BCG to BIU-87.Methods The eukaryotic expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfection Lipofectimine TM 2 000,and the expressed product was identified by immunohistochemistry S-P method and Western blot method.The effect of transfer of pCH510 into BIU-87 cells on the cytotoxicity of BCG was detected by MTT method.Results The BIU-87 cells transfected with pCH510 could express CH50 polypeptide,but the expression of CH50 polypeptide was not detectable in the control group.The positive expression of CH50 polypeptide could enhance the cytotoxicity of BCG against BIU-87 cells (P<0.05).Conclusion The expression of CH50 in BIU-87 cells could promote the antitumor function of BCG to bladder cancer cells.
出处
《临床外科杂志》
2005年第6期363-365,共3页
Journal of Clinical Surgery
基金
武汉市科技攻关课题资助项目(批准号:20036004056)
关键词
CH50多肽真核表达载体pCH510
膀胱癌细胞系BIU-87
卡介苗
转染
the eukaryotic expressing vector pCH510 of polypeptide CH50
bladder cancer cell line BIU-87
Bacillus Calmette-Guerin
gene transfection
