摘要
用聚合酶链反应（ＰＣＲ）技术对５０例心脏病患者及健康人线粒体ＤＮＡ（ｍｔＤＮＡ）中遗传物质缺失的研究发现，随年龄增长，发生缺失的ｍｔＤＮＡ比例增加。淋巴细胞和心肌细胞ｍｔＤＮＡ均存在包含有７．４３６ｋｂ缺失的片断，而前者有多重缺失，发生缺失的ｍｔＤＮＡ片断约占正常ｍｔＤＮＡ的１０．３％。提示ｍｔＤＮＡ的缺失可能在衰老和老年人心脏病的发生机制中有一定意义。
Abstract Besides nuclear DNA,mitochondrial DNA(mt DNA) is also a genetic substance in human body and may be damaged more often than nuclear DNA.With PCR amplification,the authors analyzed the 7.436kb mtDNA deletion in fifty individiuals,an age-dependent increase of mtDNA deletion was found in myocardial cells and blood lymphocytes and multiple deletion was found within lymphocyte mtDNA.The amount of deleted mtDNA was about one tenth that of normal mtDNA.It is suggested that mitochondrial DNA deletion might be a contributory factor to aging and presbycardia.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1994年第5期266-269,共4页
Chinese Journal of Geriatrics
基金
国家自然科学基金
