摘要
应用马来酸酐和苯乙烯聚合成共聚物，并将它配成丁酮－乙腈溶液，把共聚物固定到聚苯乙烯试管内壁上，借此将二抗ＩｇＧ结合到试管壁上，制备成二抗固化管．利用该管建立了固相放免分析血浆ＡＣＴＨ以及双抗固相分离血清皮质醇ＲＩＡ．所建方法的标准曲线、相关性、精密度、准确性及灵敏度都十分满意，与各自液相方法对照，测定病人样品，结果一致，两者相关密切．这些固相方法简化了操作步骤，便于推广应用．
Abstract It is difficult to immobilize antibody by the formation of covalent bonds on polystyrene tubes. As maleic anhydride-styrene copolymer is able to bind NH2groups of proteins, the copolymer was synthesized with maleic anhydride and styrene, and dissolved in solution of methylethyl ketone-acetonitrile. The polystyrene tubes (12mm × 75mm) were coated with copolymer solution and made into“activated tubes”. Aliquot of 0.5ml of the second antibody (AB2) IgG (about 300￣500μg in 0.1M citrate buffer, PH7.5) was added into each activated tube and incubated for 16 hours at room temperature, so that 30 ￣ 40μg IgG was immobilized on the surface of each activated tube, which was referred to as “AB2-immobilized tube”. Two kinds of solid phase assay have been developed with AB2-immobilized tubes. The reproducibilities of intra and interassay of these solid phase assays were quite satisfactory. Their analytical recoveries falled in the range from 94.9% to 114.3%.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1994年第3期170-172,共3页
Chinese Journal of Nuclear Medicine
