摘要
〔目的〕建立PEDV的新型套式RT-PCR检测方法,为该病的诊断和流行病学研究提供更为敏感和可靠的手段。〔方法〕根据Genbank已发表的猪流行性腹泻病毒(PEDV)N蛋白的基因序列,设计合成两对引物,在优化RT-PCR反应条件的基础上,建立了检测PEDV的新型套式RT-PCR方法,并用该方法对PEDV进行了快速诊断和分析。〔结果〕外引物扩增目的片段长度为1328bp,内引物扩增目的片段为540bp。〔结论〕该套式RT-PCR方法比普通RT-PCR更加灵敏、可靠,可用于PEDV的快速诊断和流行病学调查。
Two pairs of primers were designed according to the N gene sequence of Porcine epidemic diarrhea virus in Genbank. PCR amplification product of outer-primers is 1328bp, and the inter-primers amplification product is 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV Diagnosis and epidemiological investigation.
出处
《检验检疫科学》
2005年第3期7-9,共3页
Inspection and Quarantine Science
