摘要
笔者用盲肠结扎穿孔（ＣＬＰ）方法制作败血症大白鼠模型，观察发病过程中大鼠肝线粒体呼吸功能、线粒体各细胞色素和丙二醛（ＭＤＡ）含量、线粒体膜流动性的变化，以及它们之间的相互关系。结果显示：①ＣＬＰ１２小时，肝线粒体呼吸调节比（ＲＣＲ）较对照值下降１９．８％（Ｐ＜０．００１）。②与对照值相比，ＣＬＰ１２小时各细胞色素ａ、ｂ、ｃ１和ｃ分别下降为２８．７％（Ｐ＜０．０１），２４．８％（Ｐ＜０．０１），４２％（Ｐ＜０．０１）和２１．６％（Ｐ＜０．０１）。各细胞色素含量的减少与ＲＣＲ的降低呈正相关。③ＣＬＰ后线粒体膜的荧光偏振度（Ｐ）、各向异性（γ）、微粒度（η）均明显升高。④线粒体内ＭＤＡ含量呈进行性增高。实验资料提示：ＣＬＰ败血症早期，大鼠肝线粒体出现严重氧化磷酸化功能障碍，线粒体呼吸链上电子传递受阻、脂质过氧化反应增强和线粒体膜流动性降低可能是线粒体损伤的重要原因。
This study was designed to investigate changes of hepatic mitochondrial respiratory function, the contents of intramitochondrial cytochromes and malodialdehyde (MDA), membrane fluidity of mitochondria and their relationship following sepsis induced by cecal ligation and puncture (CLP) in rats. It was found that at 12h post CLP, respiratory control rate (RCR) of the hepatic mitochondria was significantly decreased compared with the control values ( P <0.001), Dramatic decreases in contents of cytochromes a, b, c 1, and c in the hepatic mitochondria were shown as well, among which c 1 decreased most markedly at early stage, c 1 and a at 20h post CLP. There was a significant positive correlation between cytochromes and RCR, Obvious increases investigated in fluorescence polarization (P), microvesicosity (η) and anisotropy (γ) of the mitochondrial membrane. The increase of γ was shown to be significantly negatively correlated with decrease of RCR. In addition, there was a progressive elevation of MDA contents after CLP, which was also shown to be significantly negatively correlated with decrease of RCR, and positively with γ. The results suggest that the oxidative phosphorylation dysfunction of the hepatic mitochondria could occur early and severely following CLP induced sepsis, which is possibly attributed to the blockage of electron transfer in the respiratory chain, and that the enhanced mitochondrial lipid peroxidation may lead to or aggrevate hepatic mitochondrial damage during the CLP induced sepsis.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1994年第2期67-69,共3页
Chinese Journal of Trauma
关键词
败血症
肝
细胞
线粒体
损伤
Experimental sepsis Liver Mitochondria Damage
