摘要
用蔗糖丙酮抽提的鼠脑Ⅳ型登革病毒抗原和金黄色萄萄球菌诱发免疫小鼠制备Ⅳ型登革病毒腹水折体建立ＩｇＭ折体捕捉－生物素亲和素－酶联免疫吸附试验（ＭＡＣ－ＢＡ－ＥＬＩＳＡ）检测５５份Ⅳ型登革热患者血清的ＩｇＭ抗体。急性期阳性率为４２．４％（１４／３３），恢复期阳性率为９５．５％（２１／２２）。在一些Ⅳ型登革热患者血清中，ＩｇＭ抗体的出现可早至第２或３病日。本试验具有较高的敏感性、特异性、快速和仅需单份血清等优点。
Immunoglobulin M antibody capture-biotin avidin-enzyme linked immunosorbentassay(MAC-BA-ELISA)was established for the detection of IgM antibodies among 55sera from patients with Dengue fever type Ⅳ with sucrose-acetone-extracted mouse brainDengue virus type Ⅳ antigen and aureus staphy-lococcus induced ascites antibodies toDengue virus type Ⅳ produced by immunized mouse.Positivity is 42.4%(14/33)inacute phase sera and 95.5%(21/22)in convalescent phase sera. The IgM antibody inthe sera appears as early as on the 2nd or 3rd days after onset of the disease from somepatients with Dengue fever type Ⅳ. This test has the advantages of relative specificity,sensitivity, rapidity and only single serais required.
出处
《中华传染病杂志》
CSCD
北大核心
1994年第2期78-81,共4页
Chinese Journal of Infectious Diseases
关键词
免疫球蛋白
登革热病毒
Dengue virus type Ⅳ :IgM antibody :MAC-BA-ELISA
