摘要
采用聚台酶链反应（ＰＣＲ）对３４例结核性脑膜炎（结脑）患者脑脊液（ＣＳＦ）进行检测，其中５份结核杆菌培养阳性的标本，ＰＣＲ检测均阳性；２９例ＣＳＦ结核杆菌培养阴性的标本，１９例ＰＣＲ检测阳性，总的阳性率为７０．５％。而作为对照的２０例非结脑患者的ＣＳＦ标本则无阳性。利用育法微量结核杆菌与对照菌测试，可以反证本方法的准确性，其符合率为１００％，提示本方法可以用于临床标本中结核杆菌ＤＮＡ的快速检测，不失为一快速，灵敏而又特异的诊断手段。
n this study,we used the PCR protocol tO detect the repetitive DNA sequences ofMycobacterium tuberculosis in cerebrospinal fluid samples of patients with tuberculousmeningitis,the amplification protocol gave positive results in 5 of 5 cases which werepositive by culture as well as in l9 of 29 cases which were nagative by culture(overdallsensitivity 70.5%).The control san1ples were obtained from the patients with cryptococcusmeningitis,pyogenic meningitis or brain tumor,of the 20 cases not a sigle one waspositive. We conclude that the sensitivity of PCR in the detection of Mycobacteriumtuberculosis DNA in clinical samples is superior to that of standard culture technique.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1994年第1期14-17,共4页
Chinese Journal of Infectious Diseases
