摘要
选用6个随机引物对淡紫拟青霉IPC菌株及其15株突变菌株的全基因组DNA进行随机多态性扩增,共扩增出114条250～2500bp的DNA片段,多态检测率为83.3%。利用UPG MA方法对扩增的DNA片段聚类分析,结果表明:供试菌株具有丰富的遗传多样性,应用RAPD技术可以快速准确地鉴定菌株是否突变及突变程度;参照突变菌株表型特性上的改变,该方法可以作为确定其遗传性上是否发生分化的依据。
The genomic DNA of IPC and its 15 mutated isolates was put through RAPD with 6 arbitrary primers. 114 DNA fragments ranged from 250 to 2500bp was amplified. Polymorphism detection rate was 83.3%. Cluster analysis of the RAPD profiles by using UPGMA displayed that the studied isolates have genetic polymorphism. The RAPD can detect the mutation and its degree quickly and accurately. It also provided proof for the genetic changes of the mutated isolates which show changes of phenotypic characteristics.
出处
《中国生物防治》
CSCD
北大核心
2005年第2期109-112,共4页
Chinese Journal of Biological Control
基金
广东省自然基金(021923)
云南省"十五"攻关项目(2001NG08)
国家烟草专卖局科技项目(110200101010)
