摘要
目的观察乙酰葛根素对大鼠急性局灶性脑缺血再灌注损伤的NO保护作用。方法采用大脑中动脉内拴线阻断法(MCAO)造成大鼠局灶性脑缺血再灌注损伤模型,测定脑纽织匀浆和血清中NO含量及NOS水平的变化,并进行病理组织学观察结果脑缺血再灌注24,48 h后,模型组脑组织匀浆中NO,NOS活性及血清中NO水平较对照组均有显著性升高(P< 0.01),给药组能降低其水平,且与模型组相比有显著性差异(P<0.05)。结论乙酰葛根素能通过降低NO毒性,发挥脑保护作用。
OBJECTIVE: To investigate the neuroprotective effect of acetylpuerarin (compound N-2211) and peurarin on focal cerebral ischemia-reperfusion injury. METHODS: The NO level and NOS activity in brain tissue and serum were measured in the rats with reversible middle cerebral artery occlusion (MCAO) without craniectomy. RESULTS: The levels of NO and NOS in brain homogenate increased significantly in ischemia-reperfusion group, compared with sham group, and there was the same change happened for the level of NO in serum. NO and NOS levels decreased in acetylpuerarin-treated groups and peurarin-treated group, compared with ischemia-reperfusion rats. At the same time, the number of living pyramidal cells in CA1 region of hippocampus increased significantly in acetylpuerarin-treated rats and peurarin-treated rats, compared with ischemia-reperfusion rats. CONCLUSION: The results suggested that the effect of acetylpuerarin and peurarin on decreasing NO production played a role on the amelioration of focal brain ischemia-reperfusion injury.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第11期829-832,共4页
Chinese Pharmaceutical Journal
