摘要
通过对棉铃虫Helicoverpaarmigera(H櫣bner)幼虫中肠氨肽酶N的克隆和测序,鉴定了1个氨肽酶N基因APN1,其cDNA序列具有3220个核苷酸,具有3042bp的开放阅读框,编码产生1014个氨基酸的蛋白质。其推定的氨基酸序列具有氨肽酶N所共有的锌结合模体HEXXHX18E和N末端20个氨基酸的疏水性信号序列,但C末端没有糖基磷酯酰肌醇(glycosylphosphatidylinositol,GPI)锚添加信号序列。该氨肽酶N的cDNA序列已提交GenBank,登录号为AY358034。
cDNAs of aminopeptidase N (APN) from Helicoverpa armigera (Hübner) were cloned and sequenced. One aminopeptidase N (APN1) was identified. This aminopeptidase N had 3 220 nucleotides with a 3 042 bp open reading frame that encodes 1 014 amino acids. The putative amino acid sequence shares the consensus zinc binding/gluzincin motif (HEXXHX (18)E) and a hydrophobic signal sequence with 20 amino acids in N-terminal region common to all aminopeptidases, but no glycosylphosphatidylinositol (GPI) anchor signal sequence in C-terminal region of APN1 was found. The sequences reported here had been deposited in the GenBank database (Accession number: AY358034).
出处
《昆虫学报》
CAS
CSCD
北大核心
2005年第3期444-449,共6页
Acta Entomologica Sinica
基金
国家自然科学基金项目(30270889)
关键词
棉铃虫
氨肽酶N
糖基磷酯酰肌醇锚
受体
Helicoverpa armigera
\% aminopeptidase N
GPI anchor
receptor
