摘要
目的评估人鼻咽癌细胞系CNE-2Z放射敏感性异质性与凋亡敏感性的相关性。方法用流式细胞仪、荧光显微镜、电镜、Western-blot检测H5和S1凋亡能力的不同及凋亡相关蛋白表达的差异。结果H5、S1两种细胞的凋亡率均随着照射后时间的延长而增加,但H5比S1高且差异有显著性(P<0.05)。结论人鼻咽癌细胞系CNE-2Z放射敏感性的异质性,与细胞受射线照射后凋亡呈正相关,对射线敏感的亚细胞系凋亡率高。
Objective To assess the relationship between radiosensitive heterogeneity and the apoptosis of human nasopharyngeal carcinoma (NPC) cell line CNE-2Z. Methods Flow cytometry, fluorescence microscopy and Western-blot were used to detect the apoptosis and apoptosis-related protein expression in subtypes H5 and S1 of NPC CNE-2Z cell line after radiation. Results The apoptosis rate of H5 and S1 cells increased with extension of after-irradiation time, but H5 had a higher apoptosisrate than S1 and the difference was of statistical significance (P<0.05).Conclusion The radiosensitive heterogeneity of human NPC cell line CNZ-2Z is positively correlated to the apoptosis of cells after irradiation.
出处
《实用肿瘤杂志》
CAS
2005年第3期210-212,共3页
Journal of Practical Oncology
关键词
鼻咽肿瘤
癌基因
辐射耐受性
凋亡
nasopharyngeal neoplasms
oncogenes
radiation tolerancy
apoptosis
