摘要
观察雷公藤单体Ｔ_４对正常人及类风湿关节炎（ＲＡ）患者外周血单个核细胞（ＰＢＭＣ）增殖的影响。将ＰＢＭＣ与不同剂量的Ｔ_４（１０、２０、３０和４０ｎｇ／ｍｌ）在ＰＨＡ刺激或不刺激下共同孵育７２ｈ，用ＭＴＴ比色法测定发现，Ｔ_４可显著抑制正常人经ＰＨＡ刺激或不刺激的ＰＢＭＣ增殖；对经ＰＨＡ刺激的ＲＡ患者的ＰＢＭＣ增殖亦有抑制作用，刺激的ＲＡ患者的ＰＢＭＣ增殖具有双向调节作用。提示Ｔ_４通过抑制ＰＢＭＣ增殖可能用于ＲＡ的治疗。其对ＲＡ患者ＰＢＭＣ增殖的双向作用的意义尚需进一步探讨。
Tripchlorolide(T4)is a single active ingredient recently isolated from Tripterygium wilfordii Hook. The effects of T4 on the proliferation of peripheral blood mononuclear cells (PBMC)of rheumatoid arthritis(RA)patients and normal subjects were studied, RA patients and sex-and agematched normal subjects(each 8 cases)were selected. PBMC were incubated with T4 of various doses(10, 20, 30 and 40 ng/ml) in the presence or absence of PHA for 72 hours. The results were as follows: T4 remarkably inhibited the proliferation of both PHA-stimulated and unstimulated PBMC of normal subjects and PHA-stimulated PBMC of RA patients as measured by MTT colormetric method.However,T4 showed a biphasic reaction in unstimulated PBMC of 3 RA patients.These results indicates that T4 would be t1seful in the therapy of RA, The significance of biphasic reaction occurring in RA patients needs to be further investigated.
出处
《中国医学科学院学报》
CSCD
北大核心
1994年第5期352-355,共4页
Acta Academiae Medicinae Sinicae
关键词
雷公藤
T4
类风湿性关节炎
PBMC
Tripchlorolide(T4)
peripheral blood mononuclear cells
arthritis
rheumatoid
