摘要
在结核分枝杆菌(Mycobacteriumtuberculosis,MTB)的感染中,树突状细胞(Dendriticcells ,DCs)的应答反应是机体起始免疫应答的关键。因此,利用双向电泳技术(Two_dimensionalelectrophoresis,2_DE)对人源树突状细胞受结核分枝杆菌H37RvATCC 2 72 94菌株感染前后的全细胞蛋白表达图谱进行差异比较和分析,发现其中产生差异的有4 5个蛋白质斑点,利用基质辅助激光解析电离串联飞行时间质谱技术对其中4个表达明显上调的蛋白质斑点进行分析鉴定,获得4个明确的肽指纹图谱,通过在数据库中检索分析,确定这4个蛋白质分别为人亚砷酸诱导ATP酶I(HumanArsenite_stimulatedATPase ,hASNA_I) ,膜联蛋白IV(AnnexinIV) ,γ_肌动蛋白(γ_actin) ,热休克蛋白2 7(Heatshockprotein2 7,HSP2 7)。上述发现有助于了解结核分枝杆菌入侵早期导致的树突状细胞蛋白质组表达变化,为深入研究结核分枝杆菌
The response of dendritic cells (DCs) plays an essential role in the initiation of immune responses followingMycobacterium tuberculosis (MTB) challenge. Two_dimensional electrophoresis(2_DE) was employed to compare the global protein patterns between human DCs infected and that uninfected with MTB H37Rv ATCC 27294 strains, and 45 protein spots were found to express differentially. Four protein spots which remarkably changed in DCs infected with MTB H37Rv ATCC 27294 strains were measured by matrix assisted laser desorption/ionization tandem time_of_flight(TOF/TOF) mass spectrometry. The data obtained from peptide mass fingerprinting were used in protein database search. Four protein spots in gel were identified as Human Arsenite_stimulated ATPase (hASNA_I), Annexin IV, γ_actin and Heat shock protein27(HSP27). These data provide insight into the changed global protein patterns of the DCs after infection and may prove useful for further study in the interaction between MTB and host.
出处
《微生物学报》
CAS
CSCD
北大核心
2005年第3期415-419,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金 ( 3 0 2 70 0 72 )~~
关键词
树突状细胞
结核分枝杆菌
蛋白质组
差异表达
双向电泳
Dendritic cells, Mycobacterium tuberculosis, Proteome, Differentially express, two_dimensional electrophoresis
