摘要
吗丙嗪(Pro)0.313,0.625和1.25μg·ml^(-1)可增强阿霉素(Dox)体外对艾氏腹水癌(EAC)的细胞毒作用;Pro 116.5,233和466 μg·ml^(-1)也可明显增加EAC细胞中的Dox含量,在S_(37)荷瘤小鼠中Pro可降低肝细胞线粒体而增加肿瘤细胞线粒体中Dox诱发的MDA含量。提示Pro的增效作用可能与其增加肿瘤细胞中Dox积聚有关,或许与MDA含量亦有关。
Using 3- ( 4, 5-dimethythiazole )-2, 5-diphenyltetrazolium bromide (MTT) method, the effect of probimane (Pro) on doxorubicin (Dox) cytotox-icity was studied. Pro 0-313, 0.625, and 1.25 μg·ml-1 potentiated cytotoxicity of Dox in Ehrlich as-cites carcinoma (EAC) cells. Incubation of EAC cells with Dox 10μg·ml-1 and Pro 116-5, 233, and 466 μg·ml-1 resulted in an increase in intracellular drug accumulation from 0.69±0.06 to 1.08±0.10 (μg/107 cells. In S37-bearing mice, Pro 23.3, 46.6, and 116. 5 μg·ml-1 enhanced the malondialdehyde (MDA) formation in tumor and liver mitochondria and decreased MDA formation in liver mitochondria. These results suggested that the increases of Dox accumulation and MDA formation in tumor cells by Pro might be the reasons for-synergistic effect of Pro on Dox cytotoxicity.
出处
《中国药理学报》
CSCD
1994年第1期56-59,共4页
Acta Pharmacologica Sinica
关键词
阿霉素
吗丙嗪
丙二醛
抗肿瘤活性
doxorubicin
probimane
malondiadehyde
cultured tumor cells
drug synergism
