摘要
【目的】观察活化巨噬细胞培养上清液对原代培养VSMCsⅠ型胶原代谢的影响。【方法】体外原代培养大鼠血管平滑肌细胞并制备活化巨噬细胞培养上清液,L鄄3H脯氨酸掺入法和RT鄄PCR检测不同浓度和作用时间的巨噬细胞培养上清液对VSMCs的胶原合成和Ⅰ型胶原mRNA表达的影响。【结果】活化巨噬细胞培养上清液促进VSMCs的胶原合成,L鄄3H脯氨酸掺入量明显增加,呈剂量相关性,r=0.48(P<0.05);Ⅰ型胶原mRNA表达随浓度和时间增加而上升,r值分别为0.58(P<0.05)和0.55(P<0.05)。【结论】活化巨噬细胞分泌促进VSMCs胶原代谢,呈时间和剂量相关性,这可能是支架植入术后血管内膜过度增生的重要机制之一。
Objective] To explore the effect of activated macrophage supernatant on collagen typeⅠsynthesis and collagen typeⅠmRNA expression of rattus vascular smooth muscle cells (VSMCs). [Methods]VSMCs were isolated from the rattus thoracic aorta. Then the macrophages from the peritoneal cavity were treated with 10 μg/mL lipopolysaccharide (LPS), and the activated supernatant was stored. Collagen synthesis and collagen typeⅠmRNA expression of SMCs were investigated by 3H-prolin incorporation and reverse transcription polymerase chain reaction (RT-PCR), respectively. [Results]The supernatant of treated macrophages had a stimulating effect on the collagen synthesis of VSMCs (r=0.48, P< 0.05). By the RT-PCR method, the supernatant could increase the expression of collagen typeⅠmRNA with dose-dependent and time-dependent effects (r=0.58, P< 0.05 and r=0.55, P< 0.05 respectively). [Conclusion]The activated macrophages secreted mediators that stimulate collagen synthesis and collagen typeⅠmRNA expression of SMCs. It could play an important role in the process of vascular restenosis.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第3期297-299,315,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金资助项目(30270417)
