摘要
背景:中缝大核是延脑腹侧中缝处的一个重要核团,在针刺镇痛、心血管活动调节、呼吸运动调节等方面具有重要的作用,但目前有关其在消化功能调节中的作用报道很少。目的:探讨中缝大核对家兔胃运动及胃电活动的调节作用。设计:以实验动物为研究对象的观察对比实验。单位:一所中医药大学的机能实验室。对象:本实验于2002-09/2003-04在黑龙江中医药大学机能实验室(省重点实验室)完成。选择健康新西兰白兔70只,体质量2.0～2.5kg,雌雄不拘,由黑龙江中医药大学动物中心(Ⅰ级)提供,实验中随机分为7组,即生理盐水组、5-羟色胺组、赛庚啶组、P物质组、P物质拮抗剂组、吗啡组、去甲肾上腺素组。方法:通过中缝大核插管,分别向中缝大核内注射微量的5-羟色胺、赛庚啶、P物质、P物质拮抗剂、吗啡和去甲肾上腺素,并利用霍尔效应原理同步描记家兔胃运动、胃电慢波的波幅与频率变化。主要观察指标:中缝大核区微量注射5-羟色胺、赛庚啶、P物质、P物质拮抗剂、吗啡及去甲肾上腺素后胃运动、胃电的变化。结果:中缝大核微量注入5-羟色胺、P物质可抑制家兔的胃运动和胃电慢波(P<0.05,P<0.01)。中缝大核微量注入赛庚啶、P物质拮抗剂后可使家兔的胃运动和胃电慢波增强(P<0.05,P<0.01)。中缝大核微量注入吗啡。
BACKGROUND:Nucleus raphe magnus(NRM) is an important nucleus located in the median raphe of ventral medulla.Many studies have been focused on the crucial role which plays in acupuncture analgesia,cardiovascular regulation, respiratory regulation and other aspects of health.However,little attention has been paid to its role in the regulation of digestive system heretofore. OBJECTIVE:To investigate the effect of NRM on gastric movement and gastric electric activity in rabbits. DESIGN:Comparative study based on the experimental animals. SETTING:A laboratory of functional medicine in a traditional Chinese medical university. PARTICIPANTS:From September 2002 to April 2004,the study was conducted in the functional medicine laboratory of Heilongjiang University of Chinese Medicine(Provincial Key Laboratory).Seventy healthy New Zealand white rabbits were used including male and female,which weighed 2 -2.5 kg on average,supplied by the Laboratory Animal Center of Heilongjiang University of Chinese Medicine(Class I).The rabbits were randomly divided into 7 groups: saline group,5-hydroxytryptamine(5-HT) group,cyproheptadine group,substance P group,substance pantagonist group,morphine group and noradrenaline group. METHODS:Through cannula,microinjection of 5-HT,cyproheptadine,substance P,substance P antagonist,morphine and noradrenaline were given into NRM.Synchronously,gastric movement and changes in the amplitude and frequency of gastric electric slow wave in rabbits were recorded. MAIN OUTCOME MEASUREMENTS:Gastric movement and gastric electric activity were observed after microinjection of 5-HT,cyproheptadine, substance P,substance P antagonist,morphine and noradrenaline into NRM. RESULTS:After microinjection of 5-HT and substance P into NRM, gastric movement and gastric electric slow waves in rabbits were inhibited(P< 0.05,P< 0.01).After microinjection of cyproheptadine,substance P antagonist,gastric movement and gastric electric slow waves in rabbits were increased(P< 0.05,P< 0.01). Meanwhile,it turned out that
出处
《中国临床康复》
CSCD
北大核心
2005年第15期207-209,共3页
Chinese Journal of Clinical Rehabilitation
