摘要
目的:观察抗性淀粉对2型糖尿病大鼠血糖血脂及其他生化指标的影响,为寻求适合糖尿病患者食物提供实验依据。方法:实验选用Wistar大鼠30只,随机将大鼠分为正常对照组(n=7)、糖尿病对照组(n=7)、抗性淀粉组(n=8)和普通淀粉组(n=8)。正常对照组给予常规饲料,糖尿病对照组、抗性淀粉组及普通淀粉组给予高糖高脂饲料。建立2型糖尿病大鼠模型,普通淀粉组、抗性淀粉组分别以普通玉米淀粉、马铃薯淀粉6g/(kg·d)灌胃(用蒸馏水稀释),其余两组用蒸馏水灌胃。4周后断头取血,测定各组大鼠血糖、糖化血红蛋白、胰岛素、血生化。结果:糖尿病对照组大鼠血糖、糖化血红蛋白、血脂(总胆固醇、三酰甘油)及尿酸显著高于正常对照组(P<0.05～0.01)。抗性淀粉组大鼠干预后血糖、总胆固醇和尿素氮(17.1±3.4),(4.07±0.87),(5.55±0.50)mmol/L明显低于高消化淀粉组(21.4±3.1),(4.99±1.09),(6.27±0.82)mmol/L和糖尿病对照组(21.9±3.0),(5.02±1.00),(7.72±0.81)mmol/L(P<0.05～0.01)。各组大鼠血清蛋白及钙、磷、镁水平比较,差异无显著性意义(P>0.05)。结论:抗性淀粉能降低2型糖尿病大鼠血糖血脂和尿素氮,提示抗性淀粉具有减轻糖尿病症状的作用并可能有保护肾脏功能的作用。
AIM: To observe the influence of resistant starch on blood glucose, blood lipid and other biochemical indicators in type 2 diabetic rats, so as to provide experimental evidence for seeking suitable food for diabetic patients. METHODS: Thirty Wistar rats were divided into four groups at random: normal control group (n=7), diabetic control group, resistant starch group (n=8) and digestible starch group (n=8).Rats in the normal control group were fed with command diet, while those in the diabetic control group, resistant starch group and digestible starch group were fed with high glucose and high lipid diet.After establishment of type 2 diabetic rat models, rats in the digestible starch and resistant starch groups received gastric perfusion of digestible starch and potato starch 6 g/kg per day (diluted with distilled water), while gastric perfusion of distilled water was given to those in the other two groups. The heads of the rats were cut down for blood after 4 weeks, then the levels of blood glucose,glycosylated hemoglobin (HbA1c), insulin and other biochemical indicators of rats in each group were determined. RESULTS:The levels of blood glucose, HbA1c,blood lipid (total cholesterol, triacylglycerol) and uric acid were significantly higher in the rats of the diabetic control group than in the normal control group (P< 0.05 to 0.01).The levels of blood glucose,total cholesterol and blood urea nitrogen of rats in the resistant starch group [(17.1±3.4), (4.07±0.87),(5.55±0.50) mmol/L] were obviously lower than those in the digestible starch group [(21.4±3.1), (4.99±1.09), (6.27±0.82) mmol/L] and diabetic control group [(21.9±3.0),(5.02±1.00),(7.72 ±0.81) mmol/L](P< 0.05 to 0.01).There were insignificant differences in the levels of serum protein, calcium, phosphorus and magnesium among the groups (P >0.05). CONCLUSION: Resistant starch can reduce the levels of blood glucose, blood lipid and blood urea nitrogen of type 2 diabetic rats, indicating that resistant starch has the effect of alleviating diabeti
出处
《中国临床康复》
CSCD
北大核心
2005年第15期92-93,共2页
Chinese Journal of Clinical Rehabilitation
