摘要
目的研究负载新城鸡瘟病毒修饰的自体肿瘤细胞瘤苗(NDV-ATV)抗原的树突状细胞(DCs)诱导的抗胃癌效应。方法新城鸡瘟病毒感染MNK45细胞并灭活,GMCSF、IL-4和TNF-α体外诱导扩增DCs并负载NDV修饰后的胃癌抗原,制备胃癌抗原特异性CTL;用CytoTox96TM检测其对MNK45体外杀伤效应。并以冻融胃癌细胞抗原为对照。结果负载新城鸡瘟病毒胃癌抗原DCs诱导的特异性CTL对MNK45的杀伤率达90.15%,显著高于冻融抗原的杀伤率(P<0.05)。且其对同种不同分化类型的胃癌细胞株MGC803、SGC7901也有较高的杀伤效应,而对LOVO及HepG2肿瘤细胞无显著杀伤作用(P<0.01)。结论新城鸡瘟病毒修饰的胃癌细胞,联合树突状细胞可诱导产生高效和特异的抗胃癌效应。提示NDV-ATV联合DCs可作为胃癌免疫治疗的一种有效的新方法。
Objective To explore the effect induced by dendriti c cells loaded with Newcastle disease virus-modified autologous tumor vaccine (ND V-ATV) against gastric cancer. Methods Gastric cancer cell line (MNK45) was infected with the Newcastle disease virus and then inactivated. DCs were induced from the peripher al blood mononuclear cell cultured in the presence of GM-CSF (1 000 u/ml), IL- 4 (1 000 u/ml) and TNF-α (100 ng/ml). After had been loaded with the antigens of gastric cancer modified by NDV, the DCs were used to induce the antigen speci fic CTL. The cytotoxicity of CTL to MNK45 cells in vitro was assayed by using Cy to Tox 96 TM kit, frozen-thawed gastric cancer antigens were used as contr ol. Results The killing rate of antigen specific CTL to MNK45 cells was 90.15%, which was nearly twice as high as that of control (P <0.05). Additionally, the CTL also had much higher cytotoxicity to other g astric cancer cells (MG803 and SGC7901) than that to LOVO and HepG2 cells ( P<0.05). Conclusions Efficient and specific anti- gastric cancer immuno reaction can be induced by NDV-ATV puls DCs and NDV-ATV loaded DCs might provi de a new therapy for gastric cancer.
出处
《消化外科》
CSCD
2005年第3期211-214,共4页
Journal of Digestive Surgery
关键词
胃肿瘤
免疫治疗
树突状细胞
细胞瘤苗
gastric carcinoma immunotherapy dendriti c cells tumor vaccine
