摘要
GPCRGα融合蛋白是近几年用于受体研究的新颖手段之一,它的表达确保了受体与G蛋白之间1∶1的化学计量关系、空间位置上的邻近性及适宜于高通量的配基筛选,使其为孤儿G蛋白偶联受体提供了一种新的研究策略,将在孤儿受体的配基筛选中发挥重要作用,对研发以oGPCR为作用靶点的新药产生积极意义。
GPCR-G alpha fusion protein has been used to investig ate receptors in recent years. The expression of GPCR-G alpha fusion protein en sured defined 1∶1 stoichiometry, the physical proximity of GPCR to G alpha subu nit, and was suitable for high-throughput ligand screening. The fusion protein technology provided a novel strategy to study orphan GPCRs. Obviously, it will p lay important roles in ligand screening of the orphan GPCRs and exert its signif icances in the R&D of novel drugs targeted at oGPCRs.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第5期518-521,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30171096)
全军医药卫生"十五"面上资助项目(No01MB056)
关键词
孤儿G蛋白偶联受体
G蛋白
融合蛋白
配基筛选
orphan G protein coupled receptors
G protein
fusion protein
ligand screening
