摘要
目的研究荧光定量PCR技术在法医学中的应用。方法应用Taqman技术对法医各种生物检材进行DNA定量。结果该定量PCR技术对各种法医生物检材进行了准确定量,并判断检材中是否存在抑制物,从而指导了后续STR的检验。结论定量PCR技术是法医DNA检验中一项不可缺少的辅助技术。
Objective To apply the real-time quantitative PCR assay with the TaqMan Probe to DNA analysis in Forensic science . Methods Using the TaqMan method to quantitate DNA abstracted from variety of biological samples common in Forensic science . Results The quantity of sample DNA can be obtained. Inhibiting factors in the abstracted DNA might be evaluated. And subsequent procedures of STR testing were then directed. Conclusion Real-time quantitative PCR is a necessary technique for DNA analysis in Forensic science.
出处
《中国法医学杂志》
CSCD
2005年第2期77-79,共3页
Chinese Journal of Forensic Medicine
基金
公安部基金项目(20011121401)
关键词
法医物证学
荧光定量PCR技术
定量
STR
抑制物
Forensic biological evidence
Fluorescent quantitative PCR
Quantification
STR
Inhibitor
