摘要
目的探讨再生障碍性贫血(再障)患者CD8+细胞及其组胺受体对巨核系造血的影响。方法在20例正常人的巨核细胞集落(CFU-Ck)的无血清培养体系中分别加入20例再障患者外周血CD8+细胞、再障患者外周血CD8+细胞+甲氰咪胍和单加甲氰咪胍。结果再障患者外周血CD8+细胞在体外能抑制正常人骨髓细胞CFU-Mk的生长,1.0×10-5mol/L的甲氰咪胍能解除这种抑制作用。1.0×10-5mol/L的甲氰咪胍对正常人骨髓细胞CFU-Mk的生长无抑制作用。结论再障患者CD8+细胞对正常CFU-Mk生长有明显的抑制作用,而组胺H2受体阻滞剂甲氰咪胍能解除这种抑制作用。
AIM: To investigate the effect of CD_8+ cells from aplastic anemia (AA) patients and its histamine type II (H_2) receptors on the growth of normal CFU-Mk. METHODS: The effects of CD_8+ cells and/or cimetidine, on normal human CFU-Mk growth were studied by using CFU-Mk assay. RESULTS: The CD_8+ cells from the perpheral blood of AA patients significantly suppressed the growth of normal allogeneic CFU-Mk. This inhibitory effect was blocked by cimetidine at concentration of 1.0×10^-5 mol/L. 1.0×10^-5 mol/L cimitidine alone didn't inhibit the growth of normal CFU-Mk. CONCLUSION: H_2 receptor antagonist cimitidine abolishes the suppressive effect of AA patients CD_8+ cells on the growth of normal CFU-Mk.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第5期936-938,共3页
Chinese Journal of Pathophysiology
基金
广东省卫生厅科学研究基金资助项目(No.A1998551)
深圳市科委科学研究基金资助项目(No.199805020)
