摘要
目的建立骨细粒棘球蚴病动物模型,为探索骨包虫病的发生、发展规律提供试验技术平台。方法30只长爪沙鼠,于骨膜下、骨髓腔、腰椎旁直接注射接种细粒棘球蚴混悬液,建立骨包虫病动物模型。并于感染后3、6、12个月作X线及病理检查。结果长爪沙鼠骨膜下、骨髓腔、腰椎旁接种均可感染细粒棘球蚴,感染率分别为50.00%、50.00%和16.67%。接种6个月后X片示骨膜反应;12个月放大1.64倍X光片可明确骨骼破坏情况。病理检查示,骨包虫没有包虫膜,囊肿具有外生性特征。结论成功建立骨细粒棘球蚴病长爪沙鼠动物模型,于长爪沙鼠的骨髓腔和骨膜下接种感染率较高,死亡率低;腰椎旁接种感染率偏低,且死亡率高。
Objective To establish the animal model of bone hydatid disease caused by Echinococciasis granulous, so as to investigate the law of occurrence and development of bone hydatid disease. Methods The animal mode was established by injecting E. granulous scolex gotten from liquid of sheep CE directly under periosteum, in the bone medulla canal, beside vertebral of lumbar among 30 gerbils. The gerbils were examined by X-ray at 3, 6, 12 months post infection. Results The infected rate of subperiosteum, bone medulla canal, perivertebra of lumbar was 50.00%, 50.00%, 16.67%. After 6 months of injection, the X-ray film showed the periosteum reaction. At 12 months after injection, the 1.64 scale X-ray film showed bone destruction clearly. The pathologic slide showed bone hydrated disease has no fibrous membrane out the cyst. Conclusion The infection rate of subperiosteum and bone medulla canal is high, and mortality is low. The infection rate of spine is low, and mortality is high.
出处
《中国寄生虫病防治杂志》
CSCD
2005年第2期127-128,F003,共3页
Chinese Journal of Parasitic Disease Control
关键词
骨细粒棘球蚴病
动物模型
长爪沙鼠
Echinococciasis granulous
bone hydatid disease
animal model
