摘要
本研究初步建立快速培养甘木通组培苗的体系。以幼茎段为外植体,最适的诱导愈伤组织的培养基为MS+NAA0 .1mg/L+6 -BA0 .5mg/L(单位下同);不定芽诱导和增殖的培养基为MS+NAA0 .05+6 BA0. 5,壮苗生根培养基为MS+NAA0. 1。经炼苗后,组培苗移栽成活率达78%左右。
The rapid propagation of Clematis filamentosa Dumn by tissue cluture showed that the best explant was young stem. It also showed that the callus can be easily induced in MS+NAA 0.1 mg/L+6-BA 0.5 mg/L, the medium for the buds differentiation and proliferation was MS+NAA 0.05 mg/L+6-BA 0.5 mg/L, and the medium for the root growth was MS+NAA 0.1 mg/L. The survival tube seedling can be successfully transplant.
出处
《中药材》
CAS
CSCD
北大核心
2005年第5期364-366,共3页
Journal of Chinese Medicinal Materials
