摘要
最近,由于干细胞研究普遍取得的令人鼓舞的进展以及干细胞分离、培养、移植等新技术方法的出现和发展,出生后雄性个体的生殖系干细胞———精原干细胞(spermatogonialstemcell,SSC)已成为一个愈加引人瞩目的课题。SSC位于睾丸曲细精管基膜上,既具有自增殖(self-renewal)潜能和定向分化潜能,又是自然状态下出生后个体内在整个生命期间能够进行自增殖并能将基因传递至子代的惟一成体干细胞。了解干细胞增殖与分化的调节机制,获得足够数量的种子细胞一直是所有干细胞研究共同面临的迫切问题之一。近年来,借助于SSCs移植技术这一功能性检测方法,国内外学者就SSCs体外培养的条件进行了深入探讨,取得了可喜进展。结合自己的前期工作,参考相关最新进展,就影响哺乳类SSCs体外存活、增殖、分化的因素及未来需要解决的问题进行了评述。
Recently, spermatogonial stem cell (SSC), the postnatal male germ-line stem cells, has been a subject of intense and increasing interest as a result of exciting developments in stem cell research in general and the development of new research tools allowing the isolation, culture and transplantation of these unique cells. SSC is a adult stem cell population resided in the basal compartment of the seminiferous epithelium, which is not only capable of self-renewal and differentiation, but the only adult stem cells in the normal postnatal body that undergo self-renewal throughout life and transmit gene to the progeny. In order to obtain stem cells enough for researches and application, understanding the regulation mechanisms of the proliferation and differentiation of stem cells is the common problem in stem cell research. With the aid of SSCs transplantation, a functional assay for SSCs, the culture conditions of SSCs have been examined deeply in recently years. This review focuses on what is know about the maintenance of SSCs in vitro and discusses the possible problem in future studies.
出处
《中国农学通报》
CSCD
2005年第5期16-19,23,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金(30200195,30471246)
吉林省科技发展计划项目(20010555)
军队医药卫生科研基金(01MA204)资助。
